Journal:

Article Title: Fragmentation of 23S rRNA in Strains of Proteus and Providencia Results from Intervening Sequences in the rrn (rRNA) Genes

doi:

Figure Lengend Snippet: List of strains used a

Article Snippet: P. rettgeri ATCC 14505 , 4 , 0 , 0 , 0 , 4 , 3 , 3 , 4 , 0 , 0 , 4 , 0.

Techniques:

Journal:

Article Title: Fragmentation of 23S rRNA in Strains of Proteus and Providencia Results from Intervening Sequences in the rrn (rRNA) Genes

doi:

Figure Lengend Snippet: Intervening sequences from individual genes for rRNA of selected Proteus, Providencia, and Salmonella strains. Individual rrl genes were sequenced as described in Materials and Methods, and aligned using CLUSTAL X. The conserved residues are shown by dots, and deletions are shown by spaces. The consensus sequence is at the bottom. Each sequence is identified by the strain number and the I-CeuI fragment from which it was isolated, except in the case of serovar Typhimurium, where the individual rrl gene is indicated. Letters in parentheses indicate the family of IVSs to which each sequence belongs, where a family consists of sequences with 90% or greater nucleotide identity based on DNASIS (see Tables ​Tables33 and ​and4).4). We propose four families for helix 25 and two families for helix 45. The species are as follows: Pv, Proteus vulgaris; Pp, Proteus penneri; Pm, Proteus mirabilis; Pr, Providencia rettgeri, and S. typhimurium, Salmonella serovar Typhimurium. Numbers at the end of each sequence indicate the base pairs in the IVS.

Article Snippet: P. rettgeri ATCC 14505 , 4 , 0 , 0 , 0 , 4 , 3 , 3 , 4 , 0 , 0 , 4 , 0.

Techniques: Sequencing, Isolation

Journal:

Article Title: Fragmentation of 23S rRNA in Strains of Proteus and Providencia Results from Intervening Sequences in the rrn (rRNA) Genes

doi:

Figure Lengend Snippet: Proposed secondary structure of IVSs from Proteus, Providencia, and Salmonella rRNAs. Secondary structure predictions were obtained using the mfold server available at Michael Zuker's home page (www.ibc.wustl.edu/∼zuker/). One sequence from each of family G and family D of helix 25 IVSs and of the two families of helix 45, as well as an IVS− tetraloop of helix 25 and helix 45, are shown. The structures of families E and F of helix 25, which resemble that of family G, are not shown. The helix 45 IVS from serovar Typhimurium LT2 rrlH is included. The labels show the IVS helix and family, the strain number and I-CeuI fragment from which the gene was isolated, and the species (Pr, Providencia rettgeri; Pv, Proteus vulgaris; Pm, Proteus mirabilis; and S. typhimurium LT2, Salmonella serovar Typhimurium LT2). The positions of gaps indicated by the CLUSTAL X alignment of helix 45 IVSs are shown as X to X′ and Y to Y′. The short horizontal line indicates the position at which it is postulated that the rRNA at helix 25 or helix 45 was replaced by the IVS; the boldface number above this line indicates the number of base pairs in the IVS. The residues showing conservation for either helix 25 or helix 45 from the CLUSTAL alignment in Fig. ​Fig.33 are marked by asterisks.

Article Snippet: P. rettgeri ATCC 14505 , 4 , 0 , 0 , 0 , 4 , 3 , 3 , 4 , 0 , 0 , 4 , 0.

Techniques: Sequencing, Isolation